Interaction of Vietnamese Medicinal Plant Extracts with Recombinantly Expressed Human Neurokinin-1 Receptor
http://repository.vnu.edu.vn/handle/VNU_123/11211
A primary functional receptor screening assay was performed to examine the effects of methanolic extracts from ten Vietnamese medicinal plants on the human neurokinin-1 receptor expressed from Semliki Forest virus vectors in Chinese hamster ovary cells. Extracts from Piper nigrum, Stephania cambodica and Styphnolobium japonicum were found to exert inhibition on agonist-induced human neurokinin-1 receptor activity.
Secondary assays and high performance liquid chromatography of the lead compounds addressed a possible association between pharmacological responses and these chemical compounds.
Strong inhibition of human neurokinin-1 receptor was observed for extracts revealing the highest inhibitory potency for rotundine (tetrahydropalmatine) in S. cambodica extracts with IC50 of 0.88 µM, followed by piperine and capsaicin in P. nigrum extracts with IC50 values of approximately 2 µM, whereas rutin in S. japonicum extracts failed to inhibit hNK1R up to 100 µM.
A primary functional receptor screening assay was performed to examine the effects of methanolic extracts from ten Vietnamese medicinal plants on the human neurokinin-1 receptor expressed from Semliki Forest virus vectors in Chinese hamster ovary cells. Extracts from Piper nigrum, Stephania cambodica and Styphnolobium japonicum were found to exert inhibition on agonist-induced human neurokinin-1 receptor activity.
Secondary assays and high performance liquid chromatography of the lead compounds addressed a possible association between pharmacological responses and these chemical compounds.
Strong inhibition of human neurokinin-1 receptor was observed for extracts revealing the highest inhibitory potency for rotundine (tetrahydropalmatine) in S. cambodica extracts with IC50 of 0.88 µM, followed by piperine and capsaicin in P. nigrum extracts with IC50 values of approximately 2 µM, whereas rutin in S. japonicum extracts failed to inhibit hNK1R up to 100 µM.
| Title: | Interaction of Vietnamese Medicinal Plant Extracts with Recombinantly Expressed Human Neurokinin-1 Receptor |
| Authors: | Dinh, Doan Long Pham, Thi Hong Nhung Hoang, Thi My Nhung Trinh, Tat Cuong Nguyen, Thi Hong Van Vo, Thi Thuong Lan Pham, Thanh Huyen Lundstrom, Kenneth |
| Keywords: | Piper nigrum;Stephania cambodica;Styphnolobium japonicum;Piperaceae;Fabaceae;Menispermaceae;piperine;capsaicin rotundine;rutin;neurokinin‑1 receptor;functional assays |
| Issue Date: | 2015-11-23 |
| Publisher: | Georg Thieme Verlag |
| Citation: | Long Doan Dinh, Nhung Hong Thi Pham, Nhung My Thi Hoang, Cuong Trinh Tat, Van Hong Thi Nguyen, Lan Thuong Thi Vo, Huyen Thanh Pham, Kenneth Lundstrom (2015). Interaction of Vietnamese Medicinal Plant Extracts with Recombinantly Expressed Human Neurokinin-1 Receptor. Planta Med Lett 2015; 2(01): e42-e47 |
| Abstract: | A primary functional receptor screening assay was performed to examine the effects of methanolic extracts from ten Vietnamese medicinal plants on the human neurokinin-1 receptor expressed from Semliki Forest virus vectors in Chinese hamster ovary cells. Extracts from Piper nigrum, Stephania cambodica and Styphnolobium japonicum were found to exert inhibition on agonist-induced human neurokinin-1 receptor activity. Secondary assays and high performance liquid chromatography of the lead compounds addressed a possible association between pharmacological responses and these chemical compounds. Strong inhibition of human neurokinin-1 receptor was observed for extracts revealing the highest inhibitory potency for rotundine (tetrahydropalmatine) in S. cambodica extracts with IC50 of 0.88 µM, followed by piperine and capsaicin in P. nigrum extracts with IC50 values of approximately 2 µM, whereas rutin in S. japonicum extracts failed to inhibit hNK1R up to 100 µM. |
| URI: | http://repository.vnu.edu.vn/handle/VNU_123/11211 |
| ISSN: | 2199‑157X |
| Appears in Collections: | SMP - Papers / Tham luận HN-HT |
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